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Immunology and Infection
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Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

DOI:
10.3791/57196-v

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08:52 min

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March 23, 2018

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, , , , ,
1Department of Biological Sciences,Columbia University, 2Department of Biology, Barnard College,Columbia University

Chapters

  • 00:04Title
  • 00:49Growth of Pseudomonas aeruginosa Colony Biofilms and Overlaying Colonies with Agar
  • 02:33Fixation and Sample Processing
  • 03:02Sample Embedding and Sectioning
  • 05:23Heat Fixing, Rehydration, and Mounting
  • 06:48Results: Microscopic Analysis of Microbial Colony Biofilms
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

We describe fixation, paraffin embedding, and thin sectioning techniques for microbial colony biofilms. In prepared samples, biofilm substructure and reporter expression patterns can be visualized by microscopy.

Tags

Keywords: Paraffin EmbeddingThin SectioningMicrobial Colony BiofilmsMicroscopic AnalysisGene ExpressionFluorescent ProteinBiofilm ArchitectureExopolymeric SubstancesAgar Tryptone SolutionP. AeruginosaColony BiofilmsEmbedding CassetteFixativePBSMicroscopy

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