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JoVE Journal
Biology

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Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography
 

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography

Article doi: 10.3791/57197
July 17th, 2018

Summary July 17th, 2018

This protocol describes how to visualize the transient DNA compaction in cyanobacteria. Synchronous cultivation, monitoring by fluorescence microscopy, rapid freezing, and high voltage cryo-electron tomography are used. A protocol for these methodologies is presented, and future applications and developments are discussed.

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