The protocol describes how to engineer a perfusable vascular network in a spheroid. The spheroid's surrounding microenvironment is devised to induce angiogenesis and connect the spheroid to the microchannels in a microfluidic device. The method allows the perfusion of the spheroid, which is a long-awaited technique in three-dimensional cultures.
Nashimoto, Y., Teraoka, Y., Banan Sadeghian, R., Nakamasu, A., Arima, Y., Hanada, S., Kotera, H., Nishiyama, K., Miura, T., Yokokawa, R. Perfusable Vascular Network with a Tissue Model in a Microfluidic Device. J. Vis. Exp. (134), e57242, doi:10.3791/57242 (2018).