Journal
/
Bioengineering
/
Perfusable Vascular Network with a Tissue Model in a Microfluidic Device
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

DOI:
10.3791/57242-v

•

07:05 min

•

April 04, 2018

•

, , , , , , , , ,
1Department of Micro Engineering,Kyoto University, 2Graduate School of Medical Sciences,Kyushu University, 3International Research Center for Medical Sciences (IRCMS),Kumamoto University

Chapters

  • 00:04Title
  • 00:48Polydimethylsiloxane (PDMS) Layer Fabrication and Assembly
  • 01:48Microfluidic Device Cell Seeding
  • 05:12Results: Representative Vascular Lumen Formation
  • 06:42Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol describes how to engineer a perfusable vascular network in a spheroid. The spheroid's surrounding microenvironment is devised to induce angiogenesis and connect the spheroid to the microchannels in a microfluidic device. The method allows the perfusion of the spheroid, which is a long-awaited technique in three-dimensional cultures.

Tags

Keywords: Microfluidic DevicePerfusable Vascular NetworkSpheroidHLFRFP-HUVECGFP-HUVECEndothelial MediumMaster Mix SolutionRegenerative Medicine

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57242?article_type=v

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
check_url/57242?article_type=v

A Microfluidic Device for Studying Multiple Distinct Strains
check_url/57242?article_type=v

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape
check_url/57242?article_type=v

Protocol for Biofilm Streamer Formation in a Microfluidic Device with Micro-pillars
check_url/57242?article_type=v

Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique
check_url/57242?article_type=v

Microfluidic Genipin Deposition Technique for Extended Culture of Micropatterned Vascular Muscular Thin Films
check_url/57242?article_type=v

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
check_url/57242?article_type=v

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
check_url/57242?article_type=v

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
check_url/57242?article_type=v

Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips

Read Article