Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Yuji Nashimoto; Yukako Teraoka; Ramin Banan Sadeghian; Akiko Nakamasu; Yuichiro Arima; Sanshiro Hanada; Hidetoshi Kotera; Koichi Nishiyama; Takashi Miura; Ryuji Yokokawa

doi:10.3791/57242

Journal

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Bioengineering

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Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

14,002 Views

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07:05 min

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April 04, 2018

DOI:

10.3791/57242-v

DOI:

10.3791/57242-v

•

07:05 min

April 04, 2018

•

13994 Views

Yuji Nashimoto¹, Yukako Teraoka¹, Akiko Nakamasu², Yuichiro Arima³, Sanshiro Hanada³, Hidetoshi Kotera¹, Koichi Nishiyama³, Takashi Miura², Ryuji Yokokawa¹

¹Department of Micro Engineering,Kyoto University ²Graduate School of Medical Sciences,Kyushu University ³International Research Center for Medical Sciences (IRCMS),Kumamoto University

Summary

The protocol describes how to engineer a perfusable vascular network in a spheroid. The spheroid's surrounding microenvironment is devised to induce angiogenesis and connect the spheroid to the microchannels in a microfluidic device. The method allows the perfusion of the spheroid, which is a long-awaited technique in three-dimensional cultures.