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Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients

Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients

DOI:
10.3791/57263-v

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09:16 min

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February 28, 2018

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, , , , , , , , , ,
1Vascular Biology and Regenerative Medicine Unit,Centro Cardiologico Monzino-IRCCS, 2Department of Clinical Sciences and Community Health,Università degli Studi di Milano, 3Heart Rhythm Center,Centro Cardiologico Monzino-IRCCS

Chapters

  • 00:04Title
  • 01:12Cardiac Biopsy Collection and Processing
  • 03:06Characterizing Cardiac Mesenchymal Stromal Cells (C-MSC) by Flow Cytometry
  • 05:22Oil Red O (ORO) Staining C-MSC after Adipogenic Differentiation
  • 07:02Results: Flow Cytometry Analysis and ORO Staining
  • 08:27Conclusion

Summary

Automatic Translation

Automatic Translation

In this article, a method to isolate cardiac mesenchymal stromal cells from endomyocardial bioptic samples of arrhythmogenic cardiomyopathy patients is provided. Their characterization and the protocol to boost their adipogenic differentiation are described.

Tags

Keywords: Cardiac Mesenchymal Stromal CellsArrhythmogenic CardiomyopathyEndomyocardial BiopsyAdipogenic DifferentiationPhenotypingCell IsolationCell CultureCollagenase DigestionSurface Markers

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