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Chirurgia stereotassica per la manipolazione genetica in cellule striatali di cervelli di topo neonatale
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Chirurgia stereotassica per la manipolazione genetica in cellule striatali di cervelli di topo neonatale

Article DOI: 10.3791/57270-v • 09:44 min • July 10th, 2018
July 10th, 2018 •
1, 1, 1,2
1Institute of Neuroscience, National Yang-Ming University, 2Brain Research Center, National Yang-Ming University

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Descriviamo un protocollo di chirurgia stereotassica con un dispositivo fisso testa fatti in casa per microinjecting reagenti nello striato del cervello neonatale del mouse. Questa tecnica permette la manipolazione genetica in cellule neuronali di specifiche regioni del cervello di topo neonatale.

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Problema 137 neuroscienze chirurgia stereotassica striato topi neonatali microiniezione AAV Cre loxP manipolazione del gene
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