Journal
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Immunology and Infection
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Isolation og In Vitro kultur af Murine og menneskelige alveolær makrofager
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

Isolation og In Vitro kultur af Murine og menneskelige alveolær makrofager

DOI:
10.3791/57287-v

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09:09 min

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April 20, 2018

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, , ,
1Norton Thoracic Institute,St. Joseph’s Hospital and Medical Center, 2Flow Cytometry Core,St. Joseph’s Hospital and Medical Center

Chapters

  • 00:04Title
  • 00:50Isolation of Alveolar Macrophages (AMs) from Murine Bronchoalveolar Lavage (BAL) Fluid
  • 03:58Isolation of AMs from Mouse Lung: Single-cell Suspension
  • 06:04In Vitro Culture of AMs
  • 07:06Results: Characteristics of Mouse Alveolar Macrophages
  • 07:42Conclusion

Summary

Automatic Translation

Automatic Translation

Denne meddelelse beskriver metoder til isolering og kultur af alveolær makrofager fra mennesker og murine modeller til forsøgsformål.

Tags

Alveolar MacrophagesIn Vitro CultureMurineHumanLungBronchoalveolar LavagePulmonary ImmunologyRespiratory InfectionInflammationAutoimmunityCell IsolationTracheal CatheterizationPerfusion

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