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Genetics

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Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
 

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Article DOI: 10.3791/57295-v 12:08 min March 28th, 2018
March 28th, 2018

Chapters

Summary

Using a pBIBAC-GW binary vector makes generating transgenic plants with intact single-copy insertions, an easy process. Here, a series of protocols is presented that guide the reader through the process of generating transgenic Arabidopsis plants, and testing the plants for intactness and copy number of the inserts.

Tags

Keywords: BIBAC-GW Binary Vector Gateway Cloning Transgenic Plants Single-copy Insertions Arabidopsis Transformation Agrobacterium-mediated Transformation DsRed BAR Kanamycin Resistance
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