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A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

DOI:
10.3791/57301-v

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07:25 min

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March 06, 2018

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, , , ,
1Department of Urology,Northwestern University Feinberg School of Medicine, 2The Robert H. Lurie Comprehensive Cancer Center,Northwestern University Feinberg School of Medicine, 3Department of Pathology,Northwestern University Feinberg School of Medicine

Chapters

  • 00:05Title
  • 00:59Intra-prostatic Injection
  • 04:37Results: Representative Orthotopic Murine Prostate Tumor Analyses
  • 06:17Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to demonstrate the intra-prostatic injection of prostate cancer cells, with subsequent castration. Orthotopic pre-clinical models of androgen-dependent and castration-resistant prostate cancer are critical to study the disease in the context of a clinically relevant tumor microenvironment and an immunocompetent host.

Tags

Keywords: BioluminescentFluorescentOrthotopicSyngeneicMurineProstate CancerAndrogen-dependentCastration-resistantIntra-prostatic InjectionTumor MicroenvironmentGenetic ModificationOrthotopic TumorTherapyMouseSeminal VesicleAnterior Prostate LobeMatrigelCancer Cell Solution28-gauge Needle

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