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JoVE Journal
Developmental Biology

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Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay
 

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

Article doi: 10.3791/57309
February 16th, 2018

Summary February 16th, 2018

This protocol presents a novel in vitro bead assay that more appropriately models the process of in vivo sprouting angiogenesis by incorporating pericytes. This modification enables the bead assay to more faithfully recapitulate the heterotypic cellular interactions between endothelial cells and mural cells that are critical for angiogenesis.

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