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A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals

A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals

DOI:
10.3791/57327-v

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07:39 min

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June 25, 2018

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, , , , , , , , ,
1Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization,Hunan Agriculture University, 2Texas A&M University, 3Texas A&M Institute for Genomic Medicine, 4Sun Yat-Sen Memorial Hospital

Chapters

  • 00:04Title
  • 00:49Pancreas Perfusion and Removal
  • 01:43Pancreas Digestion and Purification of Islets
  • 03:30Plating and Treatment of Islet Cells
  • 04:23ROS Staining and Measurement
  • 05:28Results: Xenobiotic Treatment-induced Reactive Oxygen Species and Inhibition of Insulin Secretion in Isolated Pancreatic Islet Cells
  • 06:39Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to isolate mouse pancreatic islet cells for screening the ROS inductions by the xenobiotics in order to identify the potential diabetogenic xenobiotic chemicals.

Tags

Keywords: PancreasIslet CellsROSType-2 DiabetesIn VitroCollagenaseDigestionIsolationPurificationGlucose-stimulated Insulin Secretion

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