A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals

Pancreas cells are sensitive target for ROS induced damages, which is associated with development of type-2 diabetes. A pancreas cell-based assays will help identify ROS inducing chemicals. The main advantage of this technique is to use isolated pancreas islet cells for simple and effective identification of the ROS inducing chemicals which may have the added diabetogenic potentials.

This method can also be used for an in vitro analysis on the effects of chemicals on a physiological function of the pancreas such as a glucose-stimulated insulin secretion. Use a pair of curved forceps to clamp the duodenum close to the position of the sphincter of Oddi. With another pair of curved forceps, clamp the duodenum wall to block the bile pathway.