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JoVE Journal
Bioengineering

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Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array
 

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Article doi: 10.3791/57377
September 7th, 2018

Summary September 7th, 2018

This article describes the detailed methodology to prepare a Multiplexed Artificial Cellular MicroEnvironment (MACME) array for high-throughput manipulation of physical and chemical cues mimicking in vivo cellular microenvironments and to identify the optimal cellular environment for human pluripotent stem cells (hPSCs) with single-cell profiling.

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