Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel

The overall goal of this procedure is to use live-cell imaging to assess cell fate decisions following treatment with the anti-mitotic drug paclitaxel. This method enables us to visualize how individual cells respond to different doses of the anti-mitotic drug paclitaxel. The main advantage of this technique is that it provides a wealth of information on single cells or whole populations that is unobtainable from fixed-cell imaging or population-based assays.

Demonstrating this method is Marc Vittoria, a MD/PhD candidate in the Ganem Laboratory. To begin, clean the glass bottom of the imaging dish with optical cleaner to remove any fingerprints or dust that may interfere with imaging. Use sufficient optical cleaner to wet the entire glass surface.