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Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
DOI:

08:29 min

May 14, 2018

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Chapters

  • 00:04Title
  • 00:39Setting Up the Microscope for Live-cell Imaging
  • 02:47Video Analysis to Identify Cell Fate in Response to Paclitaxel
  • 04:32Video Analysis to Identify Cell Fate Following Mitotic Slippage
  • 06:11Video Analysis to Identify Frequency of Nuclear Envelope Rupture
  • 07:13Results: Cell Fate Responses to Paclitaxel Treatment
  • 08:01Conclusion

Summary

Automatic Translation

Live-cell imaging provides a wealth of information on single cells or whole populations that is unattainable by fixed cell imaging alone. Here, live-cell imaging protocols to assess cell fate decisions following treatment with the anti-mitotic drug paclitaxel are described.

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