Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

During the initial steps of mineralization, cells produce extracellular matrix proteins and release matrix vesicles that accumulate calcium and phosphate to facilitate apatite nucleation. In this report, we compare the nucleation of minerals by two selected human cell models, osteoblastic hFOB 1.19 and osteosarcoma Saos-2 cells. Their mineralization profiles were analyzed by different methods.

Put all materials under the laminar, and sterilize them under UV light. Change the medium to 10 milliliters of fresh culture medium with fetal bovine serum. Resting cells are incubated without stimulators, whereas stimulated cells are treated with both ascorbic acid and beta-glycerophosphate.