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Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
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Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Article DOI: 10.3791/57423-v • 14:56 min • June 24th, 2018
June 24th, 2018 •
1, 1, 2,3,4,5,6, 2,3,4,5,6, 1, 1
1Laboratory of Lipid Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 2Université de Lyon, 3Université Lyon 1, 4L'insitut National des Sciences Appliquées (INSA) de Lyon, 5Ecole Supérieure de Chimie Physique Electronique (CPE) Lyon, 6Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche (UMR), L'institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS)

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We present a protocol to compare the state of minerals in vesicles released by two human bone cell lines: hFOB 1.19 and Saos-2. Their mineralization profiles were analyzed by Alizarin Red-S (AR-S) staining, ultraviolet (UV) light visualization, transmission electron microscopy (TEM) imaging and energy dispersive X-ray microanalysis (EDX).

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Mineral Analysis HFOB 1.19 Cells Saos-2 Cells Transmission Electron Microscopy Energy Dispersive X-ray Microanalysis Mineralization Extracellular Matrix Proteins Matrix Vesicles Apatite Nucleation Osteoblastic Cells Osteosarcoma Cells Mineralization Profiles Ascorbic Acid Beta-glycerophosphate Culture Medium Incubation Alizarin Red Staining
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