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JoVE Journal Immunology and Infection
Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
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Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration

Article DOI: 10.3791/57435-v • 11:48 min • May 7th, 2018
May 7th, 2018 •
1, 2, 1, 3, 1
1Department of Pharmacology and Systems Physiology, University of Cincinnati, 2Department of Cell & Developmental Biology, University of Michigan, 3Department of Molecular Genetics, Biochemistry & Microbiology, University of Cincinnati

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Summary
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Here we describe a protocol for establishing and culturing human- and mouse-derived 3-dimensional (3D) gastric organoids, and the method for the transfer of 3D organoids to a 2-dimensional monolayer. The use of the gastric epithelial monolayer as a novel scratch-wound assay for regeneration studies is also described.

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Mouse-derived Human-derived Primary Gastric Epithelial Monolayer Culture Regeneration Wound Healing Assay Gastric Field Gastric Epithelial Cell Regeneration Cell Migration Human Derived Gastric Organoids Helicobacter Pylori Infection Epithelial Immune Cell Co-cultures Procedure Prepared Tissue Calcium Magnesium Free DPBS
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