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March 19, 2018
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Summary
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組換えレトロ ウイルス ・ ウイルス DNA の両端を模倣した DNA オリゴマー、インタソームと呼ばれる酵素によって実行中の複合体を形成できます。Intasomes は、生化学、構造、および速度論的研究に使えます。このプロトコルは、組み立てるし、プロトタイプ フォーミー ウイルス intasomes を浄化する方法をについて説明します。
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Cite this Article
Mackler, R. M., Lopez Jr., M. A., Yoder, K. E. Assembly and Purification of Prototype Foamy Virus Intasomes. J. Vis. Exp. (133), e57453, doi:10.3791/57453 (2018).
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