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JoVE Journal
Biochemistry

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Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
 

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

Article doi: 10.3791/57470
June 23rd, 2018

Summary June 23rd, 2018

Protein aggregation elicits cellular oxidative stress. This protocol describes a method for monitoring the intracellular states of amyloidogenic proteins and the oxidative stress associated with them, using flow cytometry. The approach is used to study the behavior of soluble and aggregation-prone variants of the amyloid-β peptide.

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