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Protokol for mikroRNA overførsel i knoglemarv-afledte hæmatopoietisk stamceller til at aktivere celle Engineering kombineret med magnetisk målretning
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting
DOI:

11:37 min

June 18, 2018

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Chapters

  • 00:04Title
  • 00:53CD133 SC Isolation
  • 06:00Characterization of Freshly Isolated CD133 SCs
  • 07:21Preparation of Transfection Complexes
  • 08:26CD133 SCs Transfection and Analysis
  • 10:03Results: Successful Engineering of miR-loaded Magnetized CD133 Cells
  • 11:02Conclusion

Summary

Automatic Translation

Denne protokol illustrerer en sikker og effektiv procedure for at ændre CD133+ hæmatopoietisk stamceller. Den præsenteres ikke-virale, magnetiske polyplex-baserede tilgang kan danne grundlag for optimering af terapeutisk stamceller følger så godt som overvågning administreret celle produkt via magnetisk resonans.

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