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Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening
DOI:

05:54 min

September 05, 2018

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Chapters

  • 00:04Title
  • 00:341536-Well 3D Tumor Spheroid and 2D Cancer Cell Line Culture and Detection Reagent Addition and Analysis
  • 03:54Results: Comparing 2D- vs. 3D-Culture Compound Efficacy
  • 05:30Conclusion

Summary

Automatic Translation

Across a wide variety of disease indications, more physiologically relevant models are being developed and implemented into drug discovery programs. The new model system described here demonstrates how three-dimensional tumor spheroids can be cultured and screened in a high-throughput 1536-well plate-based system to search for new oncology drugs.

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