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An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
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Genetics
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JoVE Journal Genetics
An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

DOI:
10.3791/57494-v

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06:21 min

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May 07, 2018

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, , , ,
1Department of Biological Sciences, College of Bioscience and Biotechnology,Chungnam National University, 2Department of Pharmacology, College of Medicine,Chungnam National University, 3Department of Nursing,Gwangju Women’s University

Chapters

  • 00:05Title
  • 00:45Transient Transfection and Teb Treatment
  • 03:00Depletion of Teb
  • 04:01Results: Modulation of Transgene Expression of the pEUI() Singular Gene Switch using Teb
  • 05:11Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for the modulation of transgene expression using pEUI(+) singular gene switch by tebufenozide treatment.

Tags

Keywords: Ecdysone ReceptorGene SwitchTransgene ExpressionTebufenozideHEK 293 CellsEGFPANKRD13ATransfectionImmunoblotting

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