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Un modello di pelle sferoide 3D Organotypic Melanoma
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
A 3D Organotypic Melanoma Spheroid Skin Model
DOI:

08:49 min

May 18, 2018

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Chapters

  • 00:04Title
  • 01:20Generation of 3D Melanoma Spheroids via the Hanging Drop Method
  • 03:16Generation of the Dermal and Epidermal Counterparts of Organotypic Full Skin Reconstructs
  • 05:08Air-liquid Cultivation of Organotypic Full Skin Reconstructs and Generation of Organotypic Melanoma Spheroid Skin Reconstructs
  • 06:45Results: Validation of Organotypic Full Skin Reconstruct and Melanoma Spheroid Skin Reconstruct
  • 08:06Conclusion

Summary

Automatic Translation

Qui, presentiamo un protocollo per generare un modello di pelle 3D organotipiche melanoma sferoide che ricapitola sia l'architettura e pluricellulari complessità di un organo/del tumore in vivo ma allo stesso tempo accomoda sistematica sperimentale intervento.

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