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JoVE Journal
Genetics

This content is Open Access.

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
 

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Article doi: 10.3791/57509
August 3rd, 2018

Summary August 3rd, 2018

Next-generation sequencing (NGS) is a powerful tool for genomic characterization that is limited by the high error rate of the platform (~0.5–2.0%). We describe our methods of error-corrected sequencing that allow us to obviate the NGS error rate and detect mutations at variant allele fractions as rare as 0.0001.

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