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JoVE Journal Genetics
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Article DOI: 10.3791/57518-v • 08:37 min • April 30th, 2018
April 30th, 2018 •
*1, *1, 1, 1, 1
1Department of Pharmacology, University of Michigan
* These authors contributed equally

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Summary
Automatic Translation

Here, we present a method to engineer the genome of C. elegans using CRISPR-Cas9 ribonucleoproteins and homology dependent repair templates.

Tags

Genomic Point Mutants C. Elegans CRISPR/Cas9 Ribonucleoproteins Single Stranded Oligonucleotide Homology Directed Repair Templates Neurodegenerative Disease SgRNA Target Selection Protospacer Adjacent Motif Lyophilized SgRNA Nuclease Free TE SsODN HDR Template
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