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Biochemistry

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Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs
 

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

Article doi: 10.3791/57519
May 10th, 2018

Summary May 10th, 2018

Toeprinting aims to measure the ability of in vitro transcribed RNA to form translation initiation complexes with ribosomes under a variety of conditions. This protocol describes a method for toeprinting mammalian RNA and can be used to study both cap-dependent and IRES-driven translation.

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