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Immunology and Infection
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Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking
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Immunology and Infection
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JoVE Journal Immunology and Infection
Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking

DOI:
10.3791/57528-v

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09:01 min

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October 29, 2018

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, ,
1Department of Environmental Health,University of Cincinnati College of Medicine

Chapters

  • 00:04Title
  • 01:14Pollutant-exposed Human Monocyte-derived Dendritic Cell (DC) Sequencing
  • 04:10Imaging Flow Cytometry Analysis
  • 05:42Results: CD1d and Lamp1 Co-localization Analysis
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

Imaging flow cytometry provides an ideal approach to detect the morphological and functional alteration of cells at individual and populational levels. Disrupted endocytic function for lipid antigen presentation in pollutant-exposed human dendritic cells was demonstrated with a combined transcriptomic profiling of gene expression and morphological demonstration of protein trafficking.

Tags

Keywords: Imaging Flow CytometryTranscriptomic ProfilingCD1d TraffickingNext-generation SequencingProtein FunctionDendritic CellsSequencing Workflow

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