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JoVE Journal
Bioengineering
Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feed...
Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feed...
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications

Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications

Full Text
13,052 Views
09:08 min
August 30, 2018

DOI: 10.3791/57532-v

Ming-Cheng Chen*1, John R. Lake*1, Keith C. Heyde2, Warren C. Ruder1,2

1Department of Bioengineering,University of Pittsburgh, 2Department of Mechanical Engineering,Carnegie Mellon University

Here we present a protocol to construct a pressure-controlled syringe pump to be used in microfluidic applications. This syringe pump is made from an additively manufactured body, off-the-shelf hardware, and open-source electronics. The resulting system is low-cost, straightforward to build, and delivers well-regulated fluid flow to enable rapid microfluidic research.

This method can help enable research in any field requiring precision liquid handling including molecular, cell, and systems biology, chemical engineering and microfabrication. The main advantage of this approach is that we are able to fabricate low-cost pressure-regulated syringe pumps. This technique should save money across a wide range of disciplines.

This technique showcases how we can couple additive manufacturing with open-source electronics to make a useful piece of laboratory instrumentation. To begin, download the STL design files from the supplemental files of this publication. Prepare these files for printing by opening them in a software package dedicated to the conversion of STL model files to executable instruction sets for the 3D printer being used.

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