A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

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Cited by 5

10:56 min

September 15th, 2018

10.3791/57556-v

September 15th, 2018

7.6K views

Jiayin Yang^*¹^,²^,⁶ , Lai-Yung Wong^*² , Xiao-Yu Tian^*³ , Rui Wei¹^,² , Wing-Hon Lai² , Ka-Wing Au² , Zhiwei Luo⁴^,⁵ , Carl Ward⁴^,⁵ , Wai-In Ho² , David P. Ibañez⁴^,⁵ , Hao Liu⁴^,⁵ , Xichen Bao⁴^,⁵ , Baoming Qin⁴^,⁵ , Yu Huang³ , Miguel A. Esteban⁴^,⁵^,⁷ , Hung-Fat Tse¹^,²^,⁶^,⁷

¹Department of Medicine, University of Hong Kong-Shenzhen Hospital, ²The Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, ³School of Biomedical Sciences, Institute of Vascular Medicine, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, ⁴Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical University, ⁵Laboratory of RNA, Chromatin, and Human Disease, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, ⁶Research Centre of Heart, Brain, Hormone, and Healthy Ageing, Li Ka Shing Faculty of Medicine, University of Hong Kong, ⁷Hong Kong-Guangdong Stem Cell and Regenerative Medicine Research Centre, University of Hong Kong and Guangzhou Institutes of Biomedicine and Health

^* These authors contributed equally

Here, we present a protocol to generate a human liver chimeric mouse model of familial hypercholesterolemia using human induced pluripotent stem cell-derived hepatocytes. This is a valuable model for testing new therapies for hypercholesterolemia.

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