Journal
/
Developmental Biology
/
A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

DOI:
10.3791/57556-v

•

10:56 min

•

September 15, 2018

•

, , , , , , , , , , , , , , ,
1Department of Medicine,University of Hong Kong-Shenzhen Hospital, 2The Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine,University of Hong Kong, 3School of Biomedical Sciences, Institute of Vascular Medicine, Li Ka Shing Institute of Health Sciences,Chinese University of Hong Kong, 4Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences, Joint School of Life Sciences,Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical University, 5Laboratory of RNA, Chromatin, and Human Disease, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences, 6Research Centre of Heart, Brain, Hormone, and Healthy Ageing, Li Ka Shing Faculty of Medicine,University of Hong Kong, 7Hong Kong-Guangdong Stem Cell and Regenerative Medicine Research Centre,University of Hong Kong and Guangzhou Institutes of Biomedicine and Health

Chapters

  • 00:04Title
  • 00:53Dissociation of iHeps for Injection
  • 04:22Intrasplenic Injection of iHeps
  • 06:11Endothelial Function Test
  • 09:18Results: iHep Mediated Repopulation of LRG Mice Liver
  • 10:39Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to generate a human liver chimeric mouse model of familial hypercholesterolemia using human induced pluripotent stem cell-derived hepatocytes. This is a valuable model for testing new therapies for hypercholesterolemia.

Tags

Keywords: Familial HypercholesterolemiaHuman Liver Chimeric Mouse ModelInduced Pluripotent Stem Cell-derived HepatocytesIn Vivo Drug TestingInherited Liver DiseasesCell DissociationSingle-cell SuspensionCell Strainer

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57556?article_type=v

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
check_url/57556?article_type=v

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
check_url/57556?article_type=v

Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
check_url/57556?article_type=v

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays
check_url/57556?article_type=v

Pluripotent Stem Cell Derived Cardiac Cells for Myocardial Repair
check_url/57556?article_type=v

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)
check_url/57556?article_type=v

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
check_url/57556?article_type=v

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
check_url/57556?article_type=v

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
check_url/57556?article_type=v

Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)

Read Article