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1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research,National Institute of Environmental Health Sciences, NIH, 2Cellular and Molecular Pathology Branch, Division of the National Toxicology Program,National Institute of Environmental Health Sciences, NIH, 3Integrated Laboratory Systems Inc., 4Experimental Pathology Laboratories Inc.
Chapters
- 00:05Title
- 01:00Frozen Section Preparation for Laser Microdissection
- 04:25Polyethylene Terephthalate (PET) Membrane Slides Processing and Staining Protocol
- 06:14Laser Microdissection with UV Laser
- 11:01Results: Evaluation of RNA Quality and Downstream RNA Analysis
- 12:25Conclusion
Aqui, descrevemos um protocolo para um laser reprodutíveis captura microdissection (LCM) para isolar a malha trabecular (TM) para a análise de RNA a jusante. A capacidade de analisar as alterações na expressão gênica na TM vai ajudar na compreensão do mecanismo molecular subjacente de doenças oculares relacionadas ao TM.
