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Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
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Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Article DOI: 10.3791/57577-v • 07:49 min • August 3rd, 2018
August 3rd, 2018 •
1, 2,3, 4, 2
1David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 2Department of Respiratory Medicine, Graduate School of Medicine, The University of Tokyo, 3Hastings Center for Pulmonary Research, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, 4Department of Molecular Pathology, Graduate School of Medicine, The University of Tokyo

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Summary
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A protocol for in vitro induction of endothelial-mesenchymal transition (EndMT), which is useful for investigating cellular signaling pathways involved in EndMT, is described. In this experimental model, EndMT is induced by treatment with TGF-β in MS-1 endothelial cells.

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Molecular Analysis Endothelial-mesenchymal Transition Transforming Growth Factor-beta Signaling Vascular Biology Cancer Biology Endothelial Plasticity Pathological Conditions In Vitro EndMT Induction TGF-2 Treatment MS-1 Cells Culture Conditions Trypsinization Cell Suspension Automatic Cell Counter Long-term Culture Stimulation With TGF-2 Downstream Analysis
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