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A GPC3-targeting Bispecific Antibody, GPC3-S-Fab, with Potent Cytotoxicity
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Cancer Research
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JoVE Journal Cancer Research
A GPC3-targeting Bispecific Antibody, GPC3-S-Fab, with Potent Cytotoxicity

A GPC3-targeting Bispecific Antibody, GPC3-S-Fab, with Potent Cytotoxicity

DOI:
10.3791/57588-v

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11:13 min

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July 12, 2018

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, , , , , ,
1School of Pharmaceutical Sciences,Sun Yat-Sen University, 2Center for Cellular & Structural Biology,Sun Yat-Sen University

Chapters

  • 00:04Title
  • 00:40GPC3-S-Fab Periplasmic Purification
  • 04:00Cytotoxic Assay
  • 08:32Results: Study of the Construction and Functional Importance of Bispecific Antibody GPC3-S-Fab
  • 10:10Conclusion

Summary

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Automatic Translation

Here, we present a protocol to produce a bispecific antibody GPC3-S-Fab in Escherichia coli. The purified GPC3-S-Fab has potent cytotoxicity against GPC3 positive liver cancer cells.

Tags

Keywords: Bispecific AntibodyGPC3-S-FabE. ColiPeriplasmic FractionNickel-NTA AgaroseCytotoxicity

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