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Plant-Microbe Interaction
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Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates

Article DOI: 10.3791/57606-v • 08:59 min • July 2nd, 2018
July 2nd, 2018 •
1, 1, 1
1Department of Molecular Genetics, University of Groningen

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The goal of the protocol presented here is to study the transcriptomic response of endosphere-isolated Bacillus mycoides to potato root exudates. This method facilitates the identification of important bacterial genes involved in plant-microbe interactions and is in principle applicable to other endophytes and plants, with minor adjustments.

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Plant-microbe Interaction Transcriptional Response Bacillus Mycoides Potato Root Exudates Bacterial Strains Endosphere Rhizosphere Versatile Method Bacteria Genes Short Period Potato Surface Sterile Water 70% Ethanol 3% Sodium Hypochlorite Germinating Potato Tubers Plastic Pots Engraftment Baskets Vermiculite Autoclaving Glass Fiber Box Climate Chamber
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