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Developmental Biology

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JoVE Journal Developmental Biology
Effektiv generasjon av bukspyttkjertelen/tolvfingertarmen Homeobox Protein 1+ bakre Foregut/Pancreatic Progenitors fra hPSCs i vedheft kulturer
Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells
Journal (Developmental Biology)
Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells
A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
Journal (Developmental Biology)
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Effektiv generasjon av bukspyttkjertelen/tolvfingertarmen Homeobox Protein 1+ bakre Foregut/Pancreatic Progenitors fra hPSCs i vedheft kulturer

Article DOI: 10.3791/57641-v • 08:32 min • March 27th, 2019
March 27th, 2019 •
1, 1, 1, 1
1Center for iPS Cell Research and Application (CiRA), Kyoto University

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Her presenterer vi en detaljert protokoll for å skille menneskelig pluripotent stamceller (hPSCs) i bukspyttkjertelen/tolvfingertarmen homeobox protein 1+ (PDX1+) celler for generering av bukspyttkjertelen overleveringslinjer basert på ikke-kolonien type monolayer av dissosiert enkeltceller. Denne metoden er egnet for produksjon av homogene hPSC-avledet celler, genetisk manipulasjon og screening.

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Utviklingspsykologi biologi problemet 145 bukspyttkjertelen menneskelige embryonale stamcelleforskningen menneskelige indusert pluripotent stilk cellen menneskelige pluripotent stilk cellen differensiering utvikling bukspyttkjertelen stamfar bakre foregut regenerativ medisin diabetes
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