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Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
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Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Article DOI: 10.3791/57643-v • 12:52 min • May 12th, 2018
May 12th, 2018 •
1,2, 1,2
1Division of Molecular Cell Biology, Zoological Institute, Technische Universität Braunschweig, 2Department of Cell Biology, Helmholtz Centre for Infection Research

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We describe how micro- and photomanipulation techniques such as FRAP and photoactivation enable the determination of motility parameters and the spatiotemporal dynamics of proteins within migrating cells. Experimental readouts include subcellular dynamics and turnover of motility regulators or of the underlying actin cytoskeleton.

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Micromanipulation Techniques Morphogenetic Dynamics Turnover Of Cytoskeletal Regulators Light Microscopy FRAP Photoactivation Protein Dynamics Cytoskeletal Regulation Migration Signaling Pathway Molecule Delivery Protein Manipulation Subcellular Locations Protein Effects On Cells Protein Mobility B16-F1 Cells Cell Culture Medium Trypsin EDTA Transfection
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