/
/
Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:40Defining the Kinetics of DSB Repair Complex Formation
- 03:38Defining Nuclei in Immunofluorescence Images
- 05:44Analyzing Localization to a Long-lasting Double-strand DNA Break
- 06:40Results: Co-localization of Double-strand DNA Breaks and Repair Proteins by Immunofluorescence Microscopy
- 07:46Conclusion
Repair of double-strand DNA breaks is a dynamic process, requiring not only formation of repair complexes at the breaks, but also their resolution after the lesion is addressed. Here, we use immunofluorescence microscopy for transient and long-lasting double-stranded breaks as a tool to dissect this genome maintenance mechanism.
