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An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry
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JoVE Journal Chemistry
An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

DOI:
10.3791/57660-v

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07:12 min

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July 29, 2018

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, , , ,
1Genomic Research Center,Academia Sinica, 2Department of Chemistry,National Taiwan University

Chapters

  • 00:04Title
  • 00:45Cleaning Sample Plate
  • 01:41Sample Deposition and Recrystallization (Small Amount of Sample)
  • 03:20Analysis of a 1 μL Sample
  • 04:22Mass Spectrometry Data Acquisition and Analysis
  • 05:04Results: Carbohydrate Ion Signals in Recrystallized Structures by MALDI Mass Spectrometry
  • 06:34Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for enhancing carbohydrate ion signals in MALDI mass spectrometry by reforming crystalline structures during sample preparation processes is demonstrated.

Tags

Keywords: Matrix-assisted Laser Desorption/ionization Mass SpectrometrySample PreparationCarbohydrate Ion SignalsMethanol Depressurization25-dihydroxybenzoic AcidSialyl-lewis AMaltoheptaoseCrystal Morphology

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