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Modelagem de osteossarcoma usando síndrome de Li-Fraumeni paciente-derivado induzido Pluripotent Stem Cells
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
DOI:

08:52 min

June 13, 2018

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Chapters

  • 00:04Title
  • 00:47Differentiation of Li-Fraumeni Syndrome (LFS) Patient-derived Induced Pluripotent Stem Cell (iPSC) to Mesenchymal Stem Cell (MSC) (Days 0 – 28)
  • 02:19iPSC-derived MSC Maturation (Day 28 – 45) and Differentiation of LFS MSC to Osteoblasts (Day 0 – 24)
  • 04:33Xenograft In Vivo Tumorigenesis Model Setup
  • 06:39Results: Representative LFS MSC Differentiation
  • 08:10Conclusion

Summary

Automatic Translation

Aqui, apresentamos um protocolo para a geração de pluripotentes induzidas (iPSCs) as células-tronco do paciente fibroblastos derivada de síndrome de Li-Fraumeni (LFS), diferenciação de iPSCs através de células-tronco mesenquimais (MSCs) de osteoblastos e modelagem em vivo tumorigênese usando osteoblastos paciente-derivado do LFS.

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