Journal
/
Cancer Research
/
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

DOI:
10.3791/57685-v

•

08:32 min

•

June 07, 2018

•

, ,
1Department of Otolaryngology-Head and Neck Surgery,Baylor College of Medicine, 2Interdepartmental Graduate Program in Translational Biology and Molecular Medicine,Baylor College of Medicine

Chapters

  • 00:04Title
  • 00:55Tumor Harvest and Digestion
  • 03:02Immune and Tumor Cellular Fraction Separation and Labeling
  • 05:57Results: Representative Tumor Component Features
  • 07:27Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a method to separate and enrich components of the tumor immune and non-immune microenvironment in established subcutaneous tumors. This technique allows for the separate analysis of tumor immune infiltrate and non-immune tumor fractions which can permit comprehensive characterization of the tumor immune microenvironment.

Tags

Keywords: Tumor Immune MicroenvironmentTumor Non-immune MicroenvironmentImmune OncologyTumor ProfilingTumor DissociationTumor EnrichmentTumor Cell FractionationTumor Immune Cell SeparationCollagenaseDNaseDensity Gradient MediumSubcutaneous Tumors

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57685?article_type=v

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics
check_url/57685?article_type=v

A Murine Orthotopic Bladder Tumor Model and Tumor Detection System
check_url/57685?article_type=v

Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy
check_url/57685?article_type=v

Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug Response
check_url/57685?article_type=v

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
check_url/57685?article_type=v

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model
check_url/57685?article_type=v

Murine Model of Metastatic Liver Tumors in the Setting of Ischemia Reperfusion Injury
check_url/57685?article_type=v

Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids
check_url/57685?article_type=v

Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity
check_url/57685?article_type=v

Modeling Primary Bone Tumors and Bone Metastasis with Solid Tumor Graft Implantation into Bone

Read Article