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Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

DOI:
10.3791/57688-v

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08:37 min

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June 19, 2018

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, ,
1Sanford Burnham Prebys Medical Discovery Institute, 2Department of Bioengineering,University of California at San Diego

Chapters

  • 00:04Title
  • 00:44Infection with Id1 Virus
  • 02:58Differentiation of hPSCs into Cryopreservable First Heart Field-like Cardiac Progenitors (FHF-L CPs)
  • 04:38FHF-L CP Differentiation into Ventricular-like Cardiomyocytes
  • 06:28Use of Activin A and Lentivirus-mediated Id1 Overexpression to Generate Ventricular-like Cardiomyocytes
  • 08:12Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a scalable method, using a simple combination of Activin A and lentivirus-mediated Id1-overexpression, to generate first heart field-like cardiac progenitors and ventricular-like cardiomyocytes from human pluripotent stem cells.

Tags

Keywords: Cardiac ProgenitorsCardiomyocytesHeart FieldPluripotent Stem CellsCell-based TherapyCardiac Disease ModelingId1 LentivirusRHO/ROCK Pathway InhibitorActivin ADifferentiation

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