Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
 

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Article doi: 10.3791/57690
June 30th, 2018 Usage Statistics

Summary June 30th, 2018

Here, we present a protocol for multi-color localization of single membrane proteins in organelles of live cells. To attach fluorophores, self-labeling proteins are used. Proteins, located in different membranes compartments of the same organelle, can be localized with a precision of ~18 nm.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter