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Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface
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Chapters
- 00:04Title
- 00:58Development of a Multispecies Community Representative of the Oral Microbiome
- 02:12Maintenance and Passaging of Caco-2 and HT29-MTX Cell Lines
- 03:20Assembly of Multicompartment Cell Model Simulating the Gut Host-microbe Interface
- 05:27Bacterial Survival Following In Vitro Gastrointestinal Transit Conditions
- 06:50Bacterial Colonization Ability Following In Vitro Gastrointestinal Transit Conditions
- 08:13Results: Viability of Synthetic Bacterial Community on In Vitro Gut Host Microbe System
- 09:31Conclusion
Gut host-microbe interactions were assessed using a novel approach combining a synthetic oral community, in vitro gastrointestinal digestion, and a model of the small intestine epithelium. We present a method that can be adapted to evaluate cell invasion of pathogens and multi-species biofilms, or even to test probiotic formulations' survivability.
