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JoVE Journal Bioengineering
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A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Article DOI: 10.3791/57703-v • 11:40 min • November 14th, 2018
November 14th, 2018 •
*1, *2, 2, 3, 3, 1, 2,3, 1
1College of Natural Sciences, Chungbuk National University, 2Department of Biomedical Engineering, Inje University, 3Institute of Digital Anti-Aging Healthcare, Inje University
* These authors contributed equally

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Summary
Automatic Translation

This work provides a method for the fabrication of droplet-based microfluidic platforms and the application of polyacrylamide microspheres for microsphere-PCR amplification. The microsphere-PCR method makes it possible to obtain single-stranded DNA amplicons without separating double-stranded DNA.

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Droplet-based Microfluidic Approach Microsphere-PCR Amplification Single-stranded DNA Amplicons Combinatorial Technique Molecular Biotechnology Microfluidic Devices Asymmetry PCR Method Highly Homogenized Microspheres Single-stranded DNA Additional Separation Steps Diagnosis Therapy Ap-ta-mo-sis Assays Detection And Treatment Of Disease Three-dimensional Microfluidic System In MEMS Control Fluids Reverse Direction Visual Demonstration Microsphere Formation Flow-focusing Geometry Solidification Of Generated Microspheres Liquid PDMS Pre-polymer Base Polymer Catalyst SU-8 Mold Silicon Wafer
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