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November 14, 2018
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This work provides a method for the fabrication of droplet-based microfluidic platforms and the application of polyacrylamide microspheres for microsphere-PCR amplification. The microsphere-PCR method makes it possible to obtain single-stranded DNA amplicons without separating double-stranded DNA.
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Cite this Article
Lee, S. H., Lee, H. W., Kim, D. S., Kwon, H. G., Lee, J. H., Kim, Y., Jeong, O. C., Ahn, J. A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons. J. Vis. Exp. (141), e57703, doi:10.3791/57703 (2018).
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