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JoVE Journal Genetics
Bronchoalveolar Lavage Exosomes Lipopolysaccharide-geïnduceerde septische long letsel
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Bronchoalveolar Lavage Exosomes Lipopolysaccharide-geïnduceerde septische long letsel

Article DOI: 10.3791/57737-v • 08:27 min • May 21st, 2018
May 21st, 2018 •
1,2, 1, 1,2
1Department of Veterans Affairs, Atlanta VAMC, 2Division of Pulmonary and Critical Care Medicine, Department of Medicine, Emory University

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Muizen blootgesteld aan intraperitoneaal LPS afscheiden exosomes in Broncho-alveolaire lavage (BAL) vloeistof die zijn ingepakt met miRNAs. Met behulp van een systeem co cultuur tonen we dat exosomes vrijgegeven in de BAL vloeistof expressie van strakke junction eiwitten in bronchiale epitheliale cellen verstoren en verhogen van expressie van pro-ontsteking cytokinen die long letsel accentueren.

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Genetica kwestie 135 Exosomes microRNAs miR-155 miR-146a LPS longkanker sepsis
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