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JoVE Journal
Bioengineering

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Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
 

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Article DOI: 10.3791/57739
May 19th, 2018

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Summary May 19th, 2018

Recombinant protein-engineered hydrogels are advantageous for 3D cell culture as they allow for complete tunability of the polymer backbone and therefore, the cell microenvironment. Here, we describe the process of recombinant elastin-like protein purification and its application in 3D hydrogel cell encapsulation.

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