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Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes

DOI:
10.3791/57766-v

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08:34 min

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September 27, 2018

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, , , , , ,
12I, UVSQ, INSERM UMR1173,Université Paris-Saclay, 2Institut Pasteur,Unité de Pathogénomique Mycobactérienne

Chapters

  • 00:04Title
  • 00:46Amoebae: M. Abscessus Tn Mutant Co-culture
  • 03:32Intracellular Mycobacteria RNA Extraction for RNAseq Analysis
  • 07:18Results: Mycobacterial mRNA Extraction Analysis
  • 07:47Conclusion

Summary

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Here, we present two protocols to study Phagocyte-Mycobacterium abscessus interactions: the screening of a transposon mutant library for bacterial intracellular deficiency and the determination of bacterial intracellular transcriptome from RNA sequencing. Both approaches provide insight into the genomic advantages and transcriptomic adaptations enhancing intracellular bacteria fitness.

Tags

Keywords: Mycobacterium AbscessusVirulence MarkersIntracellular ReplicationPhagocytesMutant Library ScreeningTranscriptomic StudiesAcanthamoeba CastellaniAmoebaCo-cultureAmikacinEscherichia ColiCell LysisColumbia Agar Plates

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