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Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes
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JoVE Journal Biology
Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes

Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes

DOI:
10.3791/57768-v

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09:32 min

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February 13, 2019

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, ,
1Department of Entomology,Purdue University, 2Purdue Institute for Inflammation, Immunology and Infectious Disease,Purdue University

Chapters

  • 00:04Title
  • 00:54Larval Contact Assay
  • 02:56Adult Topical Assay
  • 05:16Adult Blood-Feeding Assay
  • 07:31Results: Validation of Larval Contact, Adult Topical, and Adult Blood-Feeding Assays to Detect Amitriptyline Toxicity in Mosquitos
  • 08:36Conclusion

Summary

Automatic Translation

Automatic Translation

Protocols are described for assessing the toxicity of chemistries to immature and adult mosquitoes for development as new classes of larvicides, adulticides and endectocides. The protocols enable high-throughput testing of multiple chemistries at single-point dose and subsequent evaluation via dose response assay to determine toxicity on contact or via ingestion.

Tags

Keywords: Toxicity TestingInsecticidal ChemistriesMosquito LarvaeMosquito AdultsVector ControlLarvicideAdulticideHigh-throughput Screening24-well PlateSerial DilutionMortality AssayGrowth ChamberScoringAdult Mosquitoes

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