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JoVE Journal
Developmental Biology

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Induction and Validation of Cellular Senescence in Primary Human Cells
 

Induction and Validation of Cellular Senescence in Primary Human Cells

Article doi: 10.3791/57782
June 20th, 2018

Summary June 20th, 2018

Here, we discuss a series of protocols for induction and validation of cellular senescence in cultured cells. We focus on different senescence-inducing stimuli and describe the quantification of common senescence-associated markers. We provide technical details using fibroblasts as a model, but the protocols can be adapted to various cellular models.

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