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A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
 

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Article doi: 10.3791/57784
August 25th, 2018

Summary August 25th, 2018

Here we present a protocol to efficiently isolate primary human keratinocytes from adult skin tissues. This method simplifies the conventional procedure by using the ROCK Inhibitor Y-27632 in the inoculation medium to spontaneously separate epidermal cells from dermal cells.

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