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Stivhet måling av myk silikon underlag for Mechanobiology studier med Widefield fluorescens mikroskop
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Stiffness Measurement of Soft Silicone Substrates for Mechanobiology Studies Using a Widefield Fluorescence Microscope
DOI:

07:02 min

July 03, 2018

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Chapters

  • 00:04Title
  • 00:42Soft Silicone Substrate Fabrication
  • 01:40Fluorescent Microbead-Soft Silicone Coupling
  • 03:34Measurement of Silicone Stiffness with Sphere Indentation
  • 04:39Silicone Stiffness Calculation (Young s Modulus)
  • 05:51Results: Representative Microbead Images and Stiffness Values
  • 06:42Conclusion

Summary

Automatic Translation

Underlag med stivhet i kilopascal-området er nyttig å studere svaret celleområde fysiologisk relevante mikro-miljø stivhet. Bruker bare widefield fluorescens mikroskop, kan den unge modulus myk silikon gelé bestemmes et innrykk med en egnet sfære.

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