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JoVE Journal
Biochemistry

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A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
 

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Article doi: 10.3791/57801
June 6th, 2018

Summary June 6th, 2018

Batch processing of yeast 2-hybrid screens allows for direct comparison of the interaction profiles of multiple bait proteins with a highly complex set of prey fusion proteins. Here, we describe refined methods, new reagents, and how to implement their use for such screens.

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