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A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
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Biochemistry
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JoVE Journal Biochemistry
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

DOI:
10.3791/57801-v

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14:23 min

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June 06, 2018

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, ,
1Molecular Physiology and Biophysics,University of Iowa

Chapters

  • 00:04Title
  • 00:45Creating Yeast Populations with Bait and Prey Library
  • 08:03Sample Preparation for DEEPN Deep Sequencing
  • 11:51Results: DEEPN Screen Allows Wide-scale Protein Interaction Studies
  • 13:00Conclusion

Summary

Automatic Translation

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Batch processing of yeast 2-hybrid screens allows for direct comparison of the interaction profiles of multiple bait proteins with a highly complex set of prey fusion proteins. Here, we describe refined methods, new reagents, and how to implement their use for such screens.

Tags

Keywords: Yeast 2-hybridProtein-protein InteractionsDeep SequencingBait PlasmidPrey LibraryMATaMATαCentromereKanamycinGal4 DNA Binding DomainImmunoblottingGenomic DNAAdapterCSM Medium

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