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Høj opløsning sammenligning af bakteriel konjugation frekvenser
JoVE Journal
Genetics
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JoVE Journal Genetics
High-Resolution Comparison of Bacterial Conjugation Frequencies
DOI:

05:18 min

January 10, 2019

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Chapters

  • 00:04Title
  • 00:28Most Probable Number (MPN) Conjugation Frequency Calculation
  • 02:37Results: Representative Probability of Donor-Initiated Conjugation Between Plasmids
  • 05:01Conclusion

Summary

Automatic Translation

Med formålet at forstå adfærd af forskellige bakterielle konjugering DNA elementer under forskellige forhold, vi beskriver en protokol til påvisning af forskelle i konjugation frekvens, med høj opløsning, til at vurdere, hvor effektivt donor bakterie initierer konjugering.

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