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1Lee Kong Chian School of Medicine,Nanyang Technological University, 2Singapore Eye Research Institute (SERI),Singapore General Hospital, 3The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet,Karolinska University Hospital, 4Cancer and Stem Cell Biology Program,Duke-NUS Medical School, 5Department of Cell and Molecular Biology,Karolinska Institutet
Chapters
- 00:04Title
- 00:33Induction of Hypoxia in HEK293A Cells
- 01:28Harvesting Cells Cultured Under Hypoxic Conditions
- 03:30Preparing Nuclear Extracts
- 05:52Immunoprecipitation of Proteins
- 07:55Results: HIF-1α Interacts with ARNT at Hypoxia
- 08:56Conclusion
Here we describe a co-immunoprecipitation protocol to study protein-protein interactions between endogenous nuclear proteins under hypoxic conditions. This method is suitable for demonstration of the interactions between transcription factors and transcriptional co-regulators at hypoxia.
